Real-time RT-PCR and western blot analysis showed that the BCR/ABL levels in miR-23-atransfected cells were lower than those in the control groups. Ectopic expression of miR-23a in K562 cells led to cellular senescence. Moreover, when K562 cells were treated with 5-aza-2′-deoxycytidine, a DNA methylation inhibitor, BCR/ABL expression was upregulated, which indicates epigenetic silencing of miR-23a in leukemic cells. BCR/ABL and miR-23a expressions were inversely related to CML, and BCR/ABL expression was regulated by miR-23a in
leukemic cells. The epigenetic silencing of miR-23a led to derepression of BCR/ABL expression, and consequently contributes Selleck LY2835219 to CML development and progression.”
“Background: Porphyromonas gingivalis has been implicated as a major pathogen in the development and progression of chronic periodontitis. P. gingivalis biofilm formation in the subgingival crevice plays an important role in the ability of the bacteria to tolerate stress signals outside the cytoplasmic membrane. Some bacteria use a distinct subfamily of sigma factors to regulate their extracytoplasmic functions (the ECF subfamily). The objective
of this study was to determine if P. gingivalis ECF sigma factors affect P. gingivalis biofilm formation. Methods: To elucidate the role of ECF sigma factors in P. gingivalis, chromosomal mutants carrying a disruption of each ECF sigma factor-encoding gene were constructed. Bacterial FK228 growth curves were measured by determining the turbidity of bacterial cultures. The quantity of biofilm growing on plates was evaluated by crystal violet staining. Results: Comparison of the growth curves of wild-type P. gingivalis strain 33277 and the ECF mutants indicated that the growth rate of the mutants was slightly lower than that of the wild-type strain. The PGN_0274- and PGN_1740-defective mutants had increased biofilm formation compared with the wild-type (p smaller than 0.001); however, the other ECF sigma factor mutants or the complemented strains did not enhance
biofilm formation. Conclusion: These results suggest that PGN_0274 and PGN_1740 play a key role in biofilm formation by P. this website gingivalis.”
“. This paper aims to demonstrate that knowledge-based hybrid learning algorithms are positioned to offer better performance in comparison with purely empirical machine learning algorithms for the automatic classification task associated with the diagnosis of a medical condition described as pulmonary embolism (PE). The main premise is that there exists substantial and significant specialized knowledge in the domain of PE, which can readily be leveraged for bootstrapping a knowledge-based hybrid classifier that employs both the explanation-based and the empirical learning.