n on rat implantation. To assess A ODNs penetrating capacity, the Hsp105 FITC ODNs was first injected into the uterine selleck chem inhibitor lumen, and then the uteri were taken for preparing sections at the indicated time points for FITC ODNs e amination by fluorescence microscopy. Strong green fluorescence representing cellu lar uptake of FITC ODNs was observed in the luminal epi thelium at 2. 5 hours after injection. A detectable fluorescence in the underlying stroma was detected 48 hours later, indicating the penetration of Hsp105 ODNs into these cells in vivo. No fluorescence was observed in the contralateral horn treated with the unla beled ODNs as the control. Based on Hsp105 e pression profile in the uterus, the time window of Hsp105 ODNs administration should be between days 3 and 5 of gestation for allowing blockage of its protein e pression.

The Inhibitors,Modulators,Libraries pregnant rat uteri were injected with either DD water, or Hsp105 S ODNs or Hsp105 A ODNs on day 3 of pregnancy, Inhibitors,Modulators,Libraries the uteri were col lected 24 h and 48 h later, and then subjected to immu nostaining analysis. As shown in Fig. 6A, an intensive staining was observed mainly in the luminal epi thelium and glandular epithelial cells in the uterus treated with water and S ODNs respectively. In contrast, the con tralateral horn treated with A ODNs showed only low level of Hsp105 staining on day 4, 24h after injection of ODNs. A marked decrease in Hsp105 immu nostaining was noted on day 5 after treatment with A ODNs. Statistical analysis by the com puter aided laser scanning densitometry showed that the Hsp105 levels between the uteri treated with DD water, S ODNs and A ODNs were significant different in the lumi nal epithelium and the glandular epithelium.

Decreasing number of implanted embryos by Inhibitors,Modulators,Libraries antisense Hsp105 ODNs treatment We further e amined whether inhibition of Hsp105 e pression could influence embryo implantation. After administration of either the antisense or the correspond ing sense Inhibitors,Modulators,Libraries Hsp105 ODNs or distilled water into the respec tive unilateral uterine horns of pregnant rats on day 3, the animals were killed on day 9, and the uteri were e amined for the number of implanted embryos as well as their morphological status. One representative picture of the A ODNs and the S ODNs treated uteri was shown. Ten and 9 embryos were observed in the S ODNs treated horns, while only 3 and 4 embryos were observed in the contralateral A ODNs treated horns.

However, all the embryos in both treated horns were nor mal by appearance and size. The water injected rats con tained eight to ten normal implanted embryos in each uterine horn in average. No significant changes Brefeldin_A in the number of implanted embryos or the embryo normality were observed in the S ODNs treated horns as compared with that in the water treated control group, indicating that the dose of ODNs used in this study was non to ic to the embryo implantation. Tofacitinib buy In contrast, as shown in Fig. 7B, a significant reduction in the number of implanted embryos in the A ODNs treated g