ons through p38, as well as the priming by IL 1B of glutamate induced calcium entry and late calcium deregulation that are prob ably involved in the e acerbation of neuronal cell damage. Therefore, the present results prompt the hypothesis that the A2AR mediated control of the priming effects of IL 1B might be a possible mechanism underlying sellekchem the striking ability of A2AR antagonists to curtail neuronal damage caused by a variety of brain insults involving glutamate induced neuroto icity and neuroinflammation. Introduction Although clinical use of stem cells has been applied to vari ous diseases, such as leukemia, Parkinson disease, diabetes, stroke, and cardiac disease, still limi tations of their clinical use e ist because of tumor formation risk, host immune rejection, and ethical issues.
However, mesenchymal stem cells are attractive compared with embryonic stem cells as a substitute resource for clin ical use. MSCs, also known as stromal progenitor cells, Inhibitors,Modulators,Libraries are found in several places in the human body, such as bone marrow, umbilical cord, umbilical cord blood, placenta, and muscle synovial membrane. Under ap propriate culture conditions, MSCs have the potential for self renewal and differentiation into various cell lineages for osteocytes, adipocytes, and chondrocytes. Recently, human umbilical cord blood or human umbilical cord tissue mesenchymal cells, iso lated from fetal origins, have been studied for clinical use because UCMSCs are considered to be a more primitive precursor than MSCs. Also, the umbilical cord matri is suggested as a better source for Inhibitors,Modulators,Libraries the MSCs than umbilical cord blood in respect of higher e pansion po tential.
Inhibitors,Modulators,Libraries The hUCMSCs were known to e press spe cific surface markers, such as CD44, CD105, CD29, CD51, SH2, and CD105, but not hematopoietic lineage markers, such as CD34, CD45, and HLA class II. Also, hUCMSCs have an immune suppressive effect or reduced immunogenicity and e press vascular endothelial growth factor and interleukin 6. Recently, UCB derived MSCs showed cytoto icity against glioma and Kaposi sar coma, and umbilical cord mesenchymal stem cells suppressed the growth of breast cancer cells. Based on previous evidence, in the present study, we in vestigated the antitumor mechanism of hUCMSCs in PC 3 prostate cancer cells and report that hUCMSCs induce antiproliferative and apoptotic effects in PC 3 cells via ac tivation of JNK and inhibition of the PI3K AKT pathway in either direct or indirect culture conditions.
Materials and methods Culture for PC 3 prostate cancer cells and hUCMSCs PC 3 prostate cancer cells were obtained from the American Type Culture Collection and maintained Inhibitors,Modulators,Libraries in RPMI1640 Carfilzomib containing 10% heat inactivated fetal bovine serum and standard antibiotics. In contrast, umbilical cord specimens especially were obtained within an hour of surgical resection under Kyung Hee Medical Center IRB approved just after appropriate written consent for the use of the human umbilical cord tissues. Human UCMSCs were isolated fro