Apoptosis ratio was analyzed
by flow cytometry. Bcl-2 and Bax and the coding mRNA were examined. It was found that the apoptosis ratio in NE group (29.4 +/- 1.8%) was significantly greater (P < 0.05) than that of the control group (10.1 +/- 1.7%). The effect of NE was attenuated by CGRP (18.7 +/- 2.1%), which was reversed by CGRP(8-37) (24.9 +/- 2.9%). NE treatment resulted in reductions in the ratio of Bcl-2/Bax (by 33%) and their mRNA (by 53%). CGRP restored the level of Bcl-2/Bax, which was abolished by CGRP(8-37). Current study suggests that norepinephrine inhibits synthesis of Bcl-2 and increases Bax and apoptosis of cardiomyocytes. CGRP restores the ratio of Bcl-2/Bax and attenuates Epigenetics inhibitor Givinostat in vitro the apoptosis induced by NE, via specific CGRP receptor. (C) 2013 Elsevier Ireland Ltd. All rights reserved.”
“Plasmalogens, 1-O-alk-1′-enyl 2-acyl glycerol phospholipids and glycolipids, seem to have evolved first in anaerobic bacteria, but they did not persist when facultative and aerobic species appeared after the concentration of oxygen increased in the early earth’s history. Later, when aerobic animal cells appeared with their mitochondria and other intracellular organelles, plasmalogen biosynthesis requiring molecular oxygen, reappeared. The possible reasons for the disappearance and reappearance
of plasmalogens in the evolution of life on earth are discussed. The sensitivity of plasmalogens
to reactive oxygen species may have caused their disappearance when respiration Idelalisib concentration first evolved. Special features of plasmalogen structure and the resulting lipid packing may account for their reappearance. (C) 2010 Elsevier Ltd. All rights reserved.”
“Increased expression of thioredoxin-interacting protein (TXNIP) has recently been proved to be a crucial event for irremediable endoplasmic reticulum (ER) stress resulting in the programmed cell death (apoptosis) of pancreatic beta-cells. The present study demonstrated that treatment with 1-10 mu g/ml tunicamycin, a potent revulsant of ER stress, drastically induced TXNIP expression accompanied by the generation of cleaved caspase-3 as an indicator of apoptosis in SK-N-SH human neuroblastoma cells. This result substantiated that TXNIP is also involved in neurodegeneration triggered by ER stress. Moreover, we evaluated the effects of nobiletin, a citrus polymethoxyflavonoid, on tunicamycin-induced apoptosis and TXNIP expression in SK-N-SH cells, because we reported previously that this flavonoid might be able to reduce TXNIP expression. Co-treatment of SK-N-SH cells with 100 mu M nobiletin and 1 mu g/ml tunicamycin for 24 h strongly suppressed apoptosis and increased TXNIP expression induced by 1 mu g/ml tunicamycin treatment alone.