The PCA analysis was also used to demonstrate which factors most significantly affected the formation of the presence of aquatic beetles in all of the analyzed samples. Only the most numerous species, which contributed more than 1 % to the total collected material, were submitted to an analysis of correlation with environmental factors. Results Physicochemical parameters of water in the studied ponds Among the physical and chemical parameters of water in ponds with different types of substrate, the ones which demonstrated statistically significant differences were distinguished (Table 2).

These are: water conductivity, HCO3 − (t test, p < 0.05), Cl−, SO4 2− (Mann–Whitney’s test, p < 0.05), followed by Ptot, Porg and BOD5 (t test, p < 0.05). The remaining parameters did not reveal any statistically this website significant differences between the given groups of ponds. Water conductivity, SO4 MI-503 ic50 2−, HCO3 − and Cl− were significantly lower in clay pits. The other parameters were higher in ponds with a gravel bottom. Characteristics of aquatic beetles The analyzed material consisted of 1976 water beetles (24.2 % of the whole collected material) (Pakulnicka 2008), which represented 87 species (Online Appendix),

that is 68 % of the species VRT752271 in vivo richness determined in all the analyzed water bodies. 78 species were found in clay pits, while gravel pits were inhabited Protirelin by 37 species. The mean values ± SD species richness (number of species S)

found in all clay pits as well as the mean value of the Shannon–Weaver index (H′) and the average number of individuals were distinctly higher than the average values determined for gravel pits. In clay pits the values were: 18.5 (±11.4), 1.7 (±1.1), 100.4 (±90.4) respectively; while gravel pits scored:5.5 (±4.1), 0.5 (±1.9), 18.5 (±18.3) respectively. The average values of the number of beetles (N), species richness (S) and the Shannon–Weaver index (H′) in ponds with different bottom material showed statistically significant differences (t test, p < 0.05). With respect to ponds which differed in plant succession stage, statistically significant differences were observed only in the mean values of species richness (S) (H = 8.79, p = 0.01) and the Shannon–Weaver index (H′) (H = 7.5, p = 0.02). Differences in the average abundance of beetles (N) between successive stages of plant succession were marginally significant (p = 0.07) (the Kruskal–Wallis test, p < 0.05). Differences in values of the mentioned indices were noticed between young ponds without aquatic vegetation and mature ponds completely overgrown with compact patches of reed. The species which were most numerous in the collected material were: Laccobius minutus (14.2 % of all beetles), Noterus crassicornis (12.7 %), Laccophilus minutus (8.3 %), Scarodytes halensis (6.