Seven plants from each experimental group were harvested at certain time intervals during the experiment, and their roots were rinsed three times in distilled water and 0.5 M EDTA. Prior to their HTS analysis each harvested plant was divided into leaves, stem and root.2.3. Laser spectrometryThe LIBS experimental setup used is shown in kinase inhibitor Gemcitabine Fig. 1. The second harmonic Inhibitors,Modulators,Libraries (532 nm) of the Nd:YAG laser system (Brilliant B, Quantel, France) was used to create the LIBS micro-plasma by focusing the laser beam with a 16 mm focal-length glass doublet (Sill Optics, Germany). The laser pulse width was ~ 5 ns and beam diameter 8 mm. The energy of the laser pulse (~10 mJ at the sample) was set and controlled by Inhibitors,Modulators,Libraries an energy meter (Field Master LM-P10, Coherent, USA).
Figure 1.The LIBS experimental setup.
1 �C Nd:YAG ablation laser, 2 �C focusing optics, 3 �C the analyzed sample, 4 �C collecting optics, 5 �C optical fiber, 6 �C monochromator, 7 �C ICCD camera, 8 �C personal …The sample was placed to the sample holder inside the ablation chamber (Tescan, Czech Inhibitors,Modulators,Libraries Republic) to the stage with precision movements (2 ��m in x, y and z direction). The LIBS analysis was performed Inhibitors,Modulators,Libraries in air on atmospheric pressure. Inhibitors,Modulators,Libraries The ablation spot was targeted and controlled for each shot by a CCD camera placed outside of the chamber. For the temporally and spectrally resolved analysis the LIBS plasma radiation was collected with quartz objectives and transported by a 3 m fiber optic system onto the entrance Inhibitors,Modulators,Libraries slit of the 0.
32 m monochromator Inhibitors,Modulators,Libraries (TRIAX 320, Jobin Yvon, France). In this study, Inhibitors,Modulators,Libraries the grating 2400 g/mm of the monochromator and 50 ��m entrance slit were used.
As a detector an ICCD camera (Horiba, Jobin Yvon, France) was employed. The camera was triggered by the Q-switch signal of the laser.2.4. Automated spectrometric measurementsSpectrometric GSK-3 measurements were carried using an automated chemical analyser BS-200 (Mindray, China). Reagents and samples were placed on cooled sample holder (4 ��C) and automatically pipetted directly into plastic cuvettes. Incubation proceeded at 37��C. Mixture was consequ
Nucleic acid bases and some of their derivatives can be detected by stripping analysis with mercury electrodes.
Brefeldin_A Using cathodic stripping voltammetry (CSV) in connection with cyclic voltammetry (CV) or differential pulse voltammetry (DPV), we were able to detect purine and pyrimidine bases at very low concentrations cisplatin dna [1]. Voltammetric ultra-trace determination of some nucleic bases in the presence of Cu(II) using a mercury electrode or solid amalgam Vismodegib GDC-0449 electrodes has been also described [2-6]. Determination is based on the formation of purine-copper complex at the electrode surface and subsequent stripping resulting in a voltammetric signal suitable for analytical purposes [3,7,8].