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in genetically resistant and susceptible mice with severe combined immunodeficiency. Am J Trop Med Hyg 1992,47(5):605–613.PubMed Competing interests Authors of this manuscript have no competing financial or personal interests or relatioships with any organization. Authors’ contributions NP and KC designed the research; KC and MA conducted the experiments; NP, KC and SWB analyzed and interpreted data; and KC and NP wrote the paper. All authors read and approved the manuscript.”
“Background Molecular diagnosis of fungal diseases has become increasingly more used in clinical GBA3 laboratories and new species morphologically similar to Aspergillus fumigatus were surprisingly revealed [1, 2]. Section Fumigati includes fungal species closely related to A. fumigatus that can go from the anamorphous Aspergillus species to the S3I-201 teleomorphic species of the genus Neosartorya[3]. Misidentification of fungal species within section Fumigati

was sporadically reported in some laboratories, particularly of fungal isolates afterwards identified as Aspergillus lentulus, Aspergillus viridinutans, Aspergillus fumigatiaffinis, Aspergillus fumisynnematus, Neosartorya pseudofischeri, Neosartorya hiratsukae and Neosartorya udagawae[1, 2, 4, 5]. These species present similar microscopical and macroscopical features to A. fumigatus and, therefore, molecular identification is at present recommended for the correct identification of species within section Fumigati. A set of genes, namely actin, calmodulin, internal transcribed spacer (ITS), rodlet A and/or β-tubulin, has been proposed for a correct identification of A. fumigatus and related species following sequencing analysis [3, 6]. Multilocus sequence typing (MLST) [4], random amplified polymorphic DNA [7], restriction fragment length polymorphism [8] and microsphere-based Luminex assay [9] may allow molecular identification of A. fumigatus. Recently, a practical and cheap electrophoretic strategy was described for molecular identification of A. fumigatus and distinction of the species within the section Fumigati[10].