In the inner zone cells treated with TNF a, there were no differences in the total cells in the wound or migrated cells in the wound. In the outer zone cells, there was a trend towards decreased Erlotinib mw proliferation at the edge and in the wound with TNF a treatment but these changes were not significant. TNF a treatment did not alter the total number of cells in the wound or the number of cells that had migrated but not prolifer ated in the wound. The effects of TGF b1 on inner and outer zone micro wound repair In the inner zone meniscal cells, there were no obser vable changes in cell accumulation or proliferation. For the inner zone cells, total cells and proliferated cells in the wound increased with time. No changes were observed with TGF b1 treatment in the cells that proliferated at the edge or in cells that migrated but did not prolifer ate in the wound.

On the other hand, in the outer zone cells 0. 1 ng mL TGF b1 increased cell accumulation. This concentration of TGF b1 also significantly increased the total cell number in the wound of the outer zone cells, as compared to the control and 1 ng mL TGF b1 treat ment groups. Inhibitors,Modulators,Libraries However, TGF b1 treatment of outer zone cells Inhibitors,Modulators,Libraries did not alter the percen tage of proliferated cells in the wound, proliferated cells at the edge, or the number of cells that had migrated into the wound but not proliferated. The effects of IL 1, TNF a, and TGF b1 in the presence of serum on inner and outer zone micro wound repair In the presence of serum, IL 1 and TNF a treatment of meniscal cells from both the inner and outer zones resulted in decreased accumulation Inhibitors,Modulators,Libraries of proliferated cells in the micro wound.

For inner zone cells, both IL 1 and TNF a decreased total cell numbers in the wound and the percentage of proliferated Inhibitors,Modulators,Libraries cells in the wound and at the edge, as compared to 10% serum treatment for 48 hours. In addition, IL 1 and TNF a suppressed cell proliferation in the Inhibitors,Modulators,Libraries wound and at the edge compared to TGF b1 treatment for 48 hours. Even at 24 hours, TNF a sup pressed total cells in the wound relative to TGF b1 treatment. There was an increase in the total number of inner zone cells in the wound and proliferated cells in the wound and at the edge over time. None of the tested factors affected inner zone cell migra tion into the wound in the presence of serum. In the outer zone cells, TGF b1 treatment at 48 hours significantly increased cell proliferation in the wound compared to all other treatments. In addition, TGF b1 treatment also promoted cell prolif eration at the edge. Furthermore, total cells and proliferated selleck chemicals KPT-330 cells in the wound increased over time. There was no effect on cell migra tion into the wound of outer zone cells with the differ ent factors in the presence of serum.