5 ± 0.5** (0.3;0.8) Salivary Cortisol (μg/dL) 0.305 ± 0.240 (0.212;0.399) 0.321 ± 0.311 (0.217;0.425) 0.016 ± 0.272 (-0.108;0.140) 0.270 ± 0.179 (0.179;0.361) 0.206 ± 0.131 (0.104;0.308) Selleck PF-562271 -0.064 ± 0.142 (-0.127;-0.002) RMR (24 h Kcal); n = 26 1290 ± 295 (1103;1477) 1228 ± 277 (1053;1400) -62 ± 184 (-179;55) 1335 ± 213 (1200;1470) 1352 ± 323 (1147;1557) 17 ± 260 (-148;152) RER; n = 26 0.809 ± 0.052 (0.776;0.842) 0.832 ± 0.41 (0.806;0.858) 0.023 ± 0.54 (-0.011;0.057) 0.841 ± 0.59 (0.804;0878) 0.822 ± 0.48 (0.791;0.853) -0.019 ± 0.85 (-0.073;0.035) Data are expressed

as means ± SD (95% confidence interval). Data were analyzed using a treatment X time repeated measures ANOVA * significant treatment X time interaction, p = 0.04 ** significant treatment X time interaction, p = 0.03 † treatment X time interaction, p = 0.08 Experimental Protocol see more Subjects reported to the laboratory first thing

in the morning following a 10-12 h overnight fast for RMR determination using open circuit indirect calorimetry (n = 26) and body composition assessment using air displacement via the Bod Pod® (n = 44). Following these tests, a NU7026 concentration saliva sample was taken via passive drool and later analyzed for cortisol content. Subjects were then randomly assigned in a double blind manner to one of two groups: Safflower oil (SO): 4 g/d of safflower oil (Genuine Health Corporation, Toronto, Ontario, CA) administered in 4 enteric-coated capsules (each capsule provided 1 g of cold pressed, high linoleic acid, safflower oil). Fish oil (FO): 4 g/d concentrated fish oil (o3mega extra strength, Genuine Health Corporation, Toronto, Ontario, CA)

administered in 4 enteric-coated capsules (each capsule provided 400 mg EPA and 200 mg DHA). Subjects took 2 capsules with breakfast and 2 capsules with dinner for a 6 wk period. All testing was repeated following 6 wk of supplementation. Body Composition Body composition was assessed by whole body densitometry using air displacement via the Bod Pod® (Life Measurements, Concord, CA). All testing was done in accordance with the manufacturer’s instructions as detailed elsewhere [24]. Briefly, subjects were tested wearing Roflumilast only tight fitting clothing (swimsuit or undergarments) and an acrylic swim cap. The subjects wore the exact same clothing for all testing. Thoracic gas volume was estimated for all subjects using a predictive equation integral to the Bod Pod® software. The calculated value for body density was used in the Siri equation [25] to estimate body composition. A complete body composition measurement was performed twice, and if the body fat % was within 0.05% the two tests were averaged. If the two tests were not within 0.05% agreement, a third test was performed and the average of 3 complete trials was used for all body composition variables. All testing was completed first thing in the morning following a 10 h overnight fast (water intake was allowed).