05 was regarded statistically sizeable. Pearson correlation coefficient was applied to assess the correlation involving TPC, FRAP and DPPH radical scavenging exercise. SPSS, version 18. 0 and Microsoft Excel 2007 statistical application were applied for that statistical and graphical evaluations. Final results Total phenolic written content The quantity of complete phenolics in extracts of C. sativum ranged from one. 73 0. 49 to 31. 38 2. 75 mg GAE g. In both of your plant components, the ethyl acetate extracts showed the highest TPC values of 31. 38 two. 75 mg GAE g and 24. 57 0. 70 mg GAE g. Ferric minimizing antioxidant electrical power Between C. sativum root extracts, the ethyl acetate extract had the highest FRAP value of 0. 129 0. 007 mmol g. As for your leaf and stem, highest FRAP worth was seen inside the dichloromethane extract.
DPPH radical scavenging action The leaf and stem aqueous extract selleck chemicals had the lowest IC50 worth of 1335. 0 37. seven ug ml. As for that root, the ethyl acetate extract which had highest FRAP worth, also displayed highest DPPH radical scavenging activity amongst root extracts. Correlation analyses of TPC with FRAP and DPPH radical scavenging activity The romance amongst phenolic articles with FRAP and DPPH scavenging activity of plant extracts had been evaluated by Pearson correlation analyses. A powerful and statistically sizeable favourable correlation was recognized involving the phenolic content material and FRAP values from the root though a weak positive correl ation was noticed involving its TPC and DPPH scavenging activity. As for that leaf and stem, substantial constructive correlation was observed be tween its TPC and DPPH scavenging activity.
Anti proliferative action The anti proliferative exercise of C. sativum extracts was investigated to the selleck RAD001 breast adenocarcinoma cell line, MCF 7, working with the MTT assay. Results have been expressed as 20% in hibitory concentration and 50% inhibitory concen tration. Among the five extracts analyzed from C. sativum root, the ethyl acetate extract exhibited the best antiproliferative activity with all the lowest IC50 worth of 200. 0 2. 6 ug ml. Amongst extracts with the leaf and stem, the hexane extract showed the lowest IC50 worth. As the root ethyl acetate extract displayed the very best anti proliferative exercise, subsequent analyses centered on this extract and its impact on MCF 7 cells. The ethyl acetate extract from the root showed significantly less toxicity to the non malignant human breast epithelial cell line, 184B5, with an IC50 worth of 317.
0 9. six ug ml when compared to MCF seven cells. Estimation of antioxidant enzymes Antioxidant enzyme routines in root ethyl acetate ex tract taken care of and untreated MCF 7 cells have been estimated. Taken care of cells showed expanding SOD exercise when compared to the untreated cells. The GPx exercise in treated cells enhanced from 6 9 h and after that decreased from 24 48 h though CAT ac tivity enhanced from 0 9 h and then decreased until eventually 48 h.