The coding region InDel was identified in LCT-EF90GL000008, which is annotated as an arpU family gene related to transcriptional regulators in the NR database (Additional file 1: Table S4) but not in VFDB (Virulence Factors Database). While small size InDels were found in sample LCT-EF258, we were also interested in large scale structural variations. We aligned the two samples with a reference at the nucleic acid level (see Methods for details) but did not identify any large scale SVs. The probable reason may be that the generation time was so short that the variations did not have enough
time to accumulate. Transcriptomic analysis Using gene difference expression analysis, 2,679 genes between LCT-EF90 and LCT-EF258 were detected. After filtering conditions of FDR ≤ 0.001 and RPKM Ratio ≥ 2, 1,159 genes remained. Both up-regulated and down-regulated genes were identified in this analysis. 4SC-202 mw Approximately Fosbretabulin manufacturer 123 genes were up-regulated, and 1,036 genes were down-regulated between LCT-EF90 and LCT-EF258 (Figure 3A). We found that the down-regulated genes significantly out-numbered up-regulated genes, suggesting that gene expression and metabolism were inhibited in LCT-EF258. Figure 3 Differential transcriptomic analysis. (A). Global profiling of gene expression changes. Here |log2Ratio|
was the log2ratio of LCT_EF258/LCT_EF90, and TPM was defined by tags per million.
(B). Clustered DEGs in COG between LCT-EF90 and LCT-EF258. (C). Clustered DEGs in GO between LCT-EF90 and LCT-EF258. The x-axis represents Bacterial neuraminidase the number of the genes corresponding to the GO functions. The y-axis represents GO functions. (D). Clustered DEGs in KEGG between LCT-EF90 and LCT-EF258. The x-axis represents the number of the genes corresponding to the KEGG pathways. The y-axis represents KEGG pathways. Different DEGs were enriched and clustered 5-Fluoracil purchase according to GO, COG and KEGG analyses. For COG, the up-regulated and down-regulated genes were summed and were compared with unchanged genes. The most change was annotated into the translation, ribosomal structure and biogenesis function classes (Figure 3B). For gene ontology, the DEGs that showed statistical significance (P-value ≤0.05) were the component, function and process ontologies. For LCT-EF90 and LCT-EF258, seven categories, including 601 DEGs (identical DEGs may fall into different categories), were shown to be meaningful (Figure 3C). For the KEGG functional cluster, there were eleven categories, including 283 DEGs, between LCT-EF90 and LCT-EF258. Most of the genes were annotated into three categories: purine metabolism, pyrimidine metabolism and ribosome (Figure 3D). Comparative proteomic analysis Using Protein Pilot software, 1188 proteins that appeared at least twice in three replicates were identified .