Similarly, proteome data revealed a consistent expression of 64 a

Similarly, proteome data revealed a consistent expression of 64 and 60 proteins

by the cattle and sheep MAP strains respectively. A comparison of these consistently detected transcripts and proteins revealed that, in the presence of iron, one third of the differentially regulated genes (P < 0.05) were represented both in the respective transcriptome and the proteomes of the two strains (Figure 1). Figure 1 Transcriptome and selleck chemicals llc proteome comparisons: Venn diagram showing the comparison of transcripts and proteins that were differentially expressed at a fold change of 1.5 or greater in cattle or sheep MAP strains in response to iron. One third of the genes differentially expressed in response to iron were represented in both the transcriptome and the proteome. Transcript profiles under iron-limiting conditions Under iron-limiting conditions both the MAP strains showed increased transcription of genes belonging to mycobactin synthesis

and esx-3, an essential secretory system of mycobactin biosynthesis (Additional file 1, Tables S2 – S5) [30]. C MAP showed increased transcription of genes belonging to ABC type transporter proteins, suf operon involved in Fe-S cluster assembly proteins GSK690693 research buy (MAP1187-MAP1192), fatty acid biosynthesis operon (MAP3188-MAP3190) and a pyruvate dehydrogenase operon (MAP2307c-MAP2309c) (Table 1 and Additional file 1, Table S5) suggesting that the transcriptional machinery is used to mobilize iron to maintain intracellular homeostasis. CMAP also upregulated expression of an enhanced intracellular survival gene (eis) (MAP2325), which was described as “”deletion 3″” in sheep strains of MAP [16]. Table 1 Transcript and protein expression in

cattle MAP under iron-limiting (LI) conditions   MAP ORF ID Predicted function aFold change       Protein Transcript Metabolism           MAP1587c alpha amylase 2.03 ± 0.2 2.87 ± 0.7   MAP1554c FadE33_2 (acyl-coA synthase) 1.79 ± 0.5 1.88 ± 0.8   MAP2307c pdhC alpha-keto acid dehydrogenase 1.68 ± 0.3 2.52 ± 0.4   MAP3189 FadE23 (acyl-CoA dehydrogenase) 2.41 ± 0.2 3.51 ± 1.0   MAP3694c FadE5 (acyl-CoA dehydrogenase) 1.87 ± 0.8 3.15 D-malate dehydrogenase ± 0.2 Cellular processes           MAP3701c heat shock protein 2.18 ± 0.6 2.48 ± 0.3   MAP1188 FeS assembly protein SufD 2.23 ± 1.0 2.73 ± 0.2   MAP1189 FeS assembly ATPase SufC 1.78 ± 0.5 2.03 ± 0.1   MAP4059 heat shock protein HtpX 1.48 ± 0.1 1.66 ± 0.5 Poorly characterized pathways           MAP1012c patatin-like phospholipase 1.67 ± 0.3 1.56 ± 0.3   MAP1944c iron suphur cluster biosynthesis 1.56 ± 0.9 1.66 ± 0.2   MAP2482 Glyoxalase/Bleomycin resistance 1.84 ± 0.3 2.19 ± 0.8   MAP3838c RES Milciclib chemical structure domain containing protein 1.50 ± 0.7 2.40 ± 0.2 aMAP oligoarray was used to measure gene expression whereas iTRAQ was used to quantitate protein expression in the cultures of cattle MAP strain grown in iron-replete (HI) or iron-limiting (LI) medium. Fold change for each target was calculated and represented as a log2 ratio of LI/HI.

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