Our work shows that metabolomic profiling approach is a promising screening tool for the diagnosis and stratification of HCC patients. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.004945, 1-13, 2011.”
“Our previous study indicated that aquaporin4 (AQP4) deficiency potentiated morphine analgesia, but attenuated tolerance and physical dependence induced by chronic exposure to morphine. However, the mechanisms remained to be explored. In the present study, effects of AQP4 deficiency on opioid receptor characteristics were investigated by [(3)H]-diprenorphine binding assays. In basal

condition, the K(d) values of opioid receptors increased from 0.27 +/- 0.03 nM in wild-type mice to 0.44 +/- 0.04 nM in AQP4 deficient mice. Meanwhile, the density (B x values) of opioid receptors increased from 0.40 +/- 0.04 pmol/mg protein in wild-type mice NVP-BSK805 to 0.66 +/- 0.04 pmol/mg protein in AQP4 deficient mice. After chronic morphine treatment, the affinity of opioid receptors decreased in wild-type mice, in which the Kd value increased from 0.27 +/- 0.03 nM to 0.40 +/- 0.04 nM, while no change INCB024360 research buy in the density of opioid receptors was observed. In AQP4 knockout mice, the effects of chronic morphine treatment on opioid receptors were similar to that in wild-type mice, in which the Kd values increased from 0.44 +/- 0.04 nM to 0.64 +/- 0.08 nM, whereas the density had no significant change. Taken together,

at the first time, we found that AQP4 deficiency decreased the affinity and increased the density of opioid receptors. Additionally, AQP4 deficiency did not affect chronic

morphine-induced alterations of opioid receptor characteristics. (C) Sapanisertib nmr 2009 Elsevier Ireland Ltd. All rights reserved.”
“Background The use of real-time two-dimensional B-mode ultrasound (RTUS)-aided central venous access device (CVAD) insertion has been recommended by health-care agencies, but a realistic failure rate for bedside attempts is unknown.\n\nMethods The failure rate of RTUS-aided CVAD insertion is estimated using data from adult inpatients and outpatients referred to a tertiary referral radiology department for a new CVAD insertion during the 2.5-year period ending February 29, 2008. Cannulation failure, complications, and additional fluoroscopic interventions per central vein cannulation attempt and per patient encounter were retrospectively collected and evaluated.\n\nResults Of the 2456 consecutive patient encounters, the index central vein cannulation failure rate using only RTUS and fluoroscopy was 4.8%; ultimate failure rate was 0.3%. The procedural mortality rate was 0.04%. If the index upper-body central vein cannulation failed, an ipsilateral upper-body attempt through a different central vein failed in 63.6%, whereas a contralateral upper-body attempt failed in 26.7% (p = 0.11) and a common femoral vein attempt failed in 11.