Compared to other viral vectors, it offer many advantages including relatively low pathogenicity in humans, wide host range and high replication efficiency[18, 19]. Therefore, we selected the improved plasimid pAdeasy to construct the recombined adenovirus Ad-HA117 containing HA117 gene and K562 cells were infected by Ad-HA117 to get the K562/Ad-HA117 cells with HA117 gene Nutlin-3 order expression. The infection efficiency and the multiplicity of infection (MOI) were detected by fluorescence and flow cytometry, it was found that the infection rate of adenovirus
to K562 cells increased with the adenovirus amout increased and the weak and dead cells increased obviously when MOI exceeded 100. So MOI 100 was chosen as the most suitable amount for the further researches (Table 1 and Figure 4). We also found that HA117 expressed only in the K562/Ad-HA117 cells and exogenous HA117 gene could induce K562 cells to develop drug resistance to the chemotherapeutic drugs such as adriamycin, vinblastine, mitoxantrone and etoposide. But HA117 gene had no drug-excretion function In conclusion, we constructed the recombined adenovirus Ad-HA117 which could express the novel gene HA117 and its expression could significantly increased the multi-drug
resistance of K562 cells. It indicated that HA117 is a functionally relevant multidrug resistance gene. But whether HA117 could increase the drug click here resistance of tumor cell in vivo needs further study. Acknowledgements We thank Professor Tong-Chuan He (molecular Oncology Laboratory of chicago university, USA) and Doctor for providing technical assistance and insightful discussions during the preparation of the manuscript. References 1. Estey EH: Cellular mechanisms of multidrug resistance of tumor cells. Biochemistry (Mosc) 2000, 65 (1) : 95–106. 2. Frame D: Molecular cancer therapeutics:
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