2007b; Pavlic et al. 2009a, b; Sakalidis et al. 2011). Cryptic species have also been resolved in several
other pathogenic genera using multigene analysis including Colletotrichum, Fusarium and Phyllosticta (Hyde et al. 2010; Summerell et al. 2010, 2011; Cai et al. 2011; Ko-Ko et al. 2011; Wikee et al. 2011a, b; Damm et al. 2012a, b). Conclusion and future work Our data analysis selleck indicates that the order Botryosphaeriales may comprise more families than the presently accepted Botryosphaeriaceae (Lumbsch and Huhndorf 2010). Clade B could be represented by Phyllostictaceae, while Clade A splits into three major clades, A1-A3. Clade A1 comprises Diplodia, Neodeightonia and Lasiodiplodia and is characterized by dark brown, LY2874455 in vivo septate, striate conidia. Clade A2 comprises Barriopsis, Phaeobotryon and Phaeobotryosphaeria, and characterized by dark to dark brown, aseptate or 2-septate ascospores, with or without an apiculus. Clade A3 includes Auerswaldia, Dothiorella and Spencermartinsia. In these genera the ascospores become brown inside the asci, while the conidia become brown when still attached to the conidiogenous cells. Clade A6 (Botryosphaeriaceae) which includes the family
type (Botryosphaeria dothidea) is characterized by hyaline, aseptate ascospores. We refrain from introducing new families for these clades at this stage until a larger dataset can confirm this. In this paper we have re-examined the type specimens of 15 genera of Botryosphaeriales, collected six new species from Thailand P505-15 and used 124 Botryosphaeriaceae strains with sequence data to derive a modern treatment for the order. There is however still much research to be carried out with resolution of families and genera, linkage of sexual and asexual morphs and differentiation of cryptic species. Acknowledgments We are grateful to the Directors and Curators of the following
herbaria for the loan of specimens in their keeping: BAFC, Nintedanib (BIBF 1120) BPI, IMI, K (M), LPS, PREM, S and ZT. The Mushroom Research Foundation, Bandoo District, Chiang Rai Province, Thailand is acknowledged for providing postgraduate scholarship support and facilities to JK Liu. Appreciation is extended to the Thailand Research Fund BRG528002 for supporting this work. References Abdollahzadeh J, Goltapeh EM, Javadi A, Shams-Bakhsh M, Zare R, Phillips AJL (2009) Barriopsis iraniana and Phaeobotryon cupressi: two new species of the Botryosphaeriaceae from trees in Iran. Persoonia 23:1–8PubMed Abdollahzadeh J, Javadi A, Goltapeh EM, Zare R, Phillips AJL (2010) Phylogeny and morphology of four new species of Lasiodiplodia from Iran. Persoonia 25:1–10PubMed Adesemoye AO, Eskalen A (2011) First report of Spencermartinsia viticola, Neofusicoccum australe, and N. parvum causing branch canker of citrus in California. Plant Dise 95:770–770 Alves A, Correia A, Luque J, Phillips AJL (2004) Botryosphaeria corticola, sp. nov.